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KMID : 0370220120560040217
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Yakhak Hoeji
2012 Volume.56 No. 4 p.217 ~ p.221
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Rapid Identification of Homoorientin from Phyllostachys bambusoides Leaves by HPLC On-line ABTS+ Screening Method
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Lee Kwang-Jin
Liang Chun
Yang Hye-Jin
Ma Jin-Yeul
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Abstract
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To determination of antioxidant substance homoorientin, from Phyllostachys bambusoides leaves, the ultrasonic extraction and HPLC on-line ABTS+ screening method were empolyed. Also, the various experimental variables such as the frequency and time of ultrasonic system were investigated and homoorientin was extracted efficiently at the low frequency
35 kHz and the extraction time 60 min. The values were positive peak 1574.71 (relative area, 23.67%) and negative peak 6924.34 (relative area, 1.23%), respectively. This HPLC on-line ABTS+ screening method was rapid and efficient to search for antioxidants from natural products. These results will provide a database for investigating the constituents of natural products and the resources of pharmaceutical and cosmetic products.
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KEYWORD
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HPLC on-line ABTS+ screening, Phyllostachys bambusoides leaves, antioxidant, homoorientin, ultrasonic
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